Schulz M K, Schnell L, Castro A J, Schwab M E, Kartje G L
Department of Anatomy and Cell Biology, Odense University, Denmark.
Exp Neurol. 1998 Feb;149(2):390-7. doi: 10.1006/exnr.1997.6731.
Fetal neocortical transplants placed into adult neocortical sensorimotor aspiration lesions are known to receive afferent input from the adult host rat brain. As this input is less dense than normal, the present study was designed to investigate whether neutralization of myelin-associated neurite growth inhibitors NI-35/250 might promote host derived cholinergic innervation of fetal neocortical transplants. Adult rats received unilateral sensorimotor cortical aspiration lesions, and block grafts from embryonic day 14-15 neocortical tissue were placed immediately into the lesion cavities. Mouse hybridoma cells secreting either the monoclonal antibody IN-1, which blocks neurite growth inhibitors NI-35/250, or a control antibody or medium without cells were applied in millipore filter capsules directly over the fetal graft tissue. The brains were processed 12 weeks later for the visualization of acetylcholinesterase-positive, presumptive cholinergic fibers. We found an enhancement in the cholinergic innervation of fetal grafts in the recipients treated with the antibody IN-1 both in terms of fibers growing into the graft and of density within the center of the grafts. These results indicate that myelin-associated neurite growth inhibitors are involved in the development of host-transplant connectivity in the adult brain.
已知植入成年大鼠新皮质感觉运动区抽吸性损伤部位的胎儿新皮质移植体可从成年宿主大鼠大脑接受传入输入。由于这种输入比正常情况稀疏，本研究旨在调查中和髓磷脂相关神经突生长抑制剂NI-35/250是否可能促进胎儿新皮质移植体的宿主源性胆碱能神经支配。成年大鼠接受单侧感觉运动皮质抽吸性损伤，并将来自胚胎第14 - 15天新皮质组织的块状移植物立即植入损伤腔内。分泌阻断神经突生长抑制剂NI-35/250的单克隆抗体IN-1的小鼠杂交瘤细胞，或对照抗体或无细胞的培养基，被应用于微孔滤膜胶囊中，直接覆盖在胎儿移植组织上。12周后对大脑进行处理，以观察乙酰胆碱酯酶阳性的假定胆碱能纤维。我们发现，在用抗体IN-1处理的受体中，胎儿移植物的胆碱能神经支配在向移植物生长的纤维以及移植物中心的密度方面均有所增强。这些结果表明，髓磷脂相关神经突生长抑制剂参与了成年大脑中宿主 - 移植体连接的发育。
