Mancini L, Moradi-Bidhendi N, Brandi M L, MacIntyre I
William Harvey Research Institute, St. Bartholomew's and the Royal London School of Medicine and Dendistry, United Kingdom.
Biochem Biophys Res Commun. 1998 Feb 24;243(3):785-90. doi: 10.1006/bbrc.1998.8175.
The gas radical, nitric oxide (NO), is a key signalling molecule in the cardiovascular, nervous and immune systems. Recently it has been found that it is produced by both the osteoblast and osteoclast and that it has major effects in producing osteoclast detachment and exerting a tonic inhibition of bone resorption. This detaching effect is mediated by a rapid increase in cGMP following calcium-triggered e-NOS activation during normal bone resorption. This effect is not reproduced in vitro by 8-bromo-cGMP but is seen with the newer rapidly permeant 8-pCPT-cGMP. However the inhibition of bone resorption by SIN-1 in vitro is not mediated solely by cGMP but depends on other factors still unidentified. Superoxide anions alone produces both osteoclast detachment and inhibition of resorption. Both of these actions may be mediated at least in part by peroxynitrite which has the same effect as NO alone on osteoclast detachment.
气体自由基一氧化氮（NO）是心血管、神经和免疫系统中的关键信号分子。最近发现，它由成骨细胞和破骨细胞产生，并且在促使破骨细胞脱离以及对骨吸收发挥持续性抑制作用方面具有重要影响。在正常骨吸收过程中，这种脱离效应是由钙触发的内皮型一氧化氮合酶（e-NOS）激活后cGMP迅速增加介导的。8-溴-cGMP在体外不会产生这种效应，但新型的快速渗透型8-对氯苯硫基-cGMP（8-pCPT-cGMP）则会出现这种效应。然而，SIN-1在体外对骨吸收的抑制作用并非仅由cGMP介导，而是取决于其他尚未明确的因素。单独的超氧阴离子会导致破骨细胞脱离并抑制骨吸收。这两种作用可能至少部分由过氧亚硝酸盐介导，过氧亚硝酸盐对破骨细胞脱离的作用与单独的NO相同。
