大肠杆菌降解体的RNA组分：rRNA降解的证据
RNA components of Escherichia coli degradosome: evidence for rRNA decay.
作者信息
Bessarab D A, Kaberdin V R, Wei C L, Liou G G, Lin-Chao S
机构信息
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China.
出版信息
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3157-61. doi: 10.1073/pnas.95.6.3157.
Abstract
Recently, we found that a multicomponent ribonucleolytic degradosome complex formed around RNase E, a key mRNA-degrading and 9S RNA-processing enzyme, contains RNA in addition to its protein components. Herein we show that the RNA found in the degradosome consists primarily of rRNA fragments that have a range of distinctive sizes. We further show that rRNA degradation is carried out in the degradosome by RNase E cleavage of A+U-rich single-stranded regions of mature 16S and 23S rRNAs. The 5S rRNA, which is known to be generated by RNase E processing of the 9S precursor, was also identified in the degradosome, but tRNAs, which are not cleaved by RNase E in vitro, were absent. Our results, which provide evidence that decay of mature rRNAs occurs in growing Escherichia coli cells in the RNA degradosome, implicate RNase E in degradosome-mediated decay.
摘要
最近，我们发现，在核糖核酸酶E（一种关键的mRNA降解和9S RNA加工酶）周围形成的多组分核糖核酸酶解聚体复合物，除了其蛋白质成分外还含有RNA。在此我们表明，在解聚体中发现的RNA主要由一系列具有不同大小的rRNA片段组成。我们进一步表明，rRNA降解是由核糖核酸酶E切割成熟16S和23S rRNA富含A+U的单链区域在解聚体中进行的。已知由9S前体经核糖核酸酶E加工产生的5S rRNA也在解聚体中被鉴定出来，但在体外不会被核糖核酸酶E切割的tRNA则不存在。我们的结果为成熟rRNA在生长中的大肠杆菌细胞的RNA解聚体中发生降解提供了证据，表明核糖核酸酶E参与了解聚体介导的降解过程。
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