流体静压增强了酿酒酵母中羧基荧光素或羧基二氯荧光素的活体染色：通过流式细胞术高效检测标记酵母。
Hydrostatic pressure enhances vital staining with carboxyfluorescein or carboxydichlorofluorescein in Saccharomyces cerevisiae: efficient detection of labeled yeasts by flow cytometry.
作者信息
Abe F
机构信息
DEEPSTAR Group, Japan Marine Science and Technology Center, Yokosuka, Japan.
出版信息
Appl Environ Microbiol. 1998 Mar;64(3):1139-42. doi: 10.1128/AEM.64.3.1139-1142.1998.
Abstract
The extent of intracellular accumulation of the fluorescent dye carboxyfluorescein or carboxydichlorofluorescein (CDCF) in Saccharomyces cerevisiae was found to be increased 5- to 10-fold under a nonlethal hydrostatic pressure of 30 to 50 MPa. This observation was confirmed by analysis of individual labeled cells by flow cytometry. The pressure-induced enhancement of staining with CDCF required D-glucose and was markedly inhibited by 2-deoxy-D-glucose, suggesting that glucose metabolism has a role in the process.
摘要
发现在30至50兆帕的非致死性静水压力下，酿酒酵母中荧光染料羧基荧光素或羧基二氯荧光素（CDCF）的细胞内积累程度增加了5至10倍。通过流式细胞术对单个标记细胞进行分析证实了这一观察结果。CDCF染色的压力诱导增强需要D-葡萄糖，并且被2-脱氧-D-葡萄糖显著抑制，这表明葡萄糖代谢在该过程中起作用。
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