Troussellier M, Courties C, Vaquer A
Laboratoire d'Hydrobiologie Marine,URA-CNRS 1355, Université de Montpellier II, France.
Biol Cell. 1993;78(1-2):111-21. doi: 10.1016/0248-4900(93)90121-t.
Microorganisms (unicellular algae, bacteria) constitute fundamental compartments of aquatic ecosystems because of their high concentrations and activities. The evaluation and understanding of their behavior and role raise different problems for which traditional methodologies are often inadequate, whether they refer to global or classical microscopic analyses. Flow cytometry (FCM) has been recently used to study microorganisms in aquatic environments. Although this technology is still applied on a limited scale in our field, a large number of works has been done showing that FCM seems to be a promising tool for aquatic microbial ecology. This paper summarizes, from the literature produced during the last decade and with original data obtained in our laboratory, the main questions related to the cell identification, the evaluation of cell viability, biomasses and productions and the measurements of bacterial and phytoplanktonic activities. The representatives of sampling and observation scales is also discussed within the framework of the FCM measurements.
微生物（单细胞藻类、细菌）因其高浓度和高活性而构成了水生生态系统的基本组成部分。对它们的行为和作用进行评估和理解会引发不同的问题，而传统方法往往不足以解决这些问题，无论这些方法是涉及整体分析还是经典的微观分析。流式细胞术（FCM）最近已被用于研究水生环境中的微生物。尽管这项技术在我们这个领域的应用规模仍然有限，但大量研究表明，流式细胞术似乎是水生微生物生态学中一个很有前景的工具。本文从过去十年的文献以及我们实验室获得的原始数据中进行总结，阐述了与细胞识别、细胞活力评估、生物量和产量评估以及细菌和浮游植物活性测量相关的主要问题。还在流式细胞术测量的框架内讨论了采样和观测尺度的代表性问题。
