Kataoka M, Sri Rohani L P, Wada M, Kita K, Yanase H, Urabe I, Shimizu S
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
Biosci Biotechnol Biochem. 1998 Jan;62(1):167-9. doi: 10.1271/bbb.62.167.
Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP+ and glucose, similarly to commercially available GDH.
对过量表达来自巨大芽孢杆菌IWG3的葡萄糖脱氢酶（GDH）基因的大肠杆菌JM109（pGDA2）作为辅因子再生剂的用途进行了研究。在由醛还原酶过量产生的转化体大肠杆菌JM109（pKAR）对4-氯-3-氧代丁酸乙酯的不对称还原反应中，大肠杆菌JM109（pGDA2）可以与NADP⁺和葡萄糖一起作为NADPH再生剂，类似于市售的GDH。
