在一种手性醇生产系统中作为辅因子再生体表达来自巨大芽孢杆菌的葡萄糖脱氢酶基因的大肠杆菌转化体。
Escherichia coli transformant expressing the glucose dehydrogenase gene from Bacillus megaterium as a cofactor regenerator in a chiral alcohol production system.
作者信息
Kataoka M, Sri Rohani L P, Wada M, Kita K, Yanase H, Urabe I, Shimizu S
机构信息
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
出版信息
Biosci Biotechnol Biochem. 1998 Jan;62(1):167-9. doi: 10.1271/bbb.62.167.
Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP+ and glucose, similarly to commercially available GDH.
对过量表达来自巨大芽孢杆菌IWG3的葡萄糖脱氢酶（GDH）基因的大肠杆菌JM109（pGDA2）作为辅因子再生剂的用途进行了研究。在由醛还原酶过量产生的转化体大肠杆菌JM109（pKAR）对4-氯-3-氧代丁酸乙酯的不对称还原反应中，大肠杆菌JM109（pGDA2）可以与NADP⁺和葡萄糖一起作为NADPH再生剂，类似于市售的GDH。
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