Calvin M, Dyson M, Rymer J, Young S R
Department of Anatomy and Cell Biology, Guy's Hospital, London, UK.
Br J Obstet Gynaecol. 1998 Feb;105(2):223-7. doi: 10.1111/j.1471-0528.1998.tb10057.x.
To demonstrate the effect of a deficiency of ovarian hormones on the process of wound contraction, using the oophorectomised rat model of the human menopause.
A randomised controlled trial.
Ninety-six adult Wistar rats were randomly allocated into either an oophorectomised group or a sham-oophorectomised control group.
Having confirmed a significant reduction in plasma oestradiol levels in the oophorectomised rats, full-thickness excised lesions were made in the flank skin of the adult rats at either two weeks or four months after oophorectomy, so that the effects of two different durations of hormone deficiency could be assessed and compared with the sham-oophorectomised controls. Following wounding, the rats were left for 3, 5, 10 or 22 days; wound contraction was assessed from photographs of the wounds taken at these intervals after injury.
In the rats wounded four months after oophorectomy there was a slower rate of wound contraction, resulting in larger wounds at days 3, 5, 10 and 22, compared with control rats. No significant difference was observed in rats wounded two weeks after oophorectomy, indicating that the effects of ovarian hormone deficiency on this process are delayed.
Due to the pivotal role of wound contraction in the process of wound healing these findings may be of clinical relevance and could have an important impact on the administration of hormone replacement therapy.
利用人类更年期的卵巢切除大鼠模型，证明卵巢激素缺乏对伤口收缩过程的影响。
一项随机对照试验。
96只成年Wistar大鼠被随机分为卵巢切除组或假手术卵巢切除对照组。
在确认卵巢切除大鼠血浆雌二醇水平显著降低后，于卵巢切除术后两周或四个月在成年大鼠侧腹皮肤制作全层切除伤口，以便评估激素缺乏两种不同持续时间的影响，并与假手术卵巢切除对照组进行比较。受伤后，让大鼠分别存活3、5、10或22天；根据受伤后这些时间间隔拍摄的伤口照片评估伤口收缩情况。
与对照大鼠相比，卵巢切除术后四个月受伤的大鼠伤口收缩速度较慢，在第3、5、10和22天时伤口更大。卵巢切除术后两周受伤的大鼠未观察到显著差异，表明卵巢激素缺乏对该过程的影响具有延迟性。
由于伤口收缩在伤口愈合过程中的关键作用，这些发现可能具有临床相关性，并且可能对激素替代疗法的应用产生重要影响。
