Garzón M, De Andrés I, Reinoso-Suárez F
Departamento de Morfología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Neuroscience. 1998 Apr;83(4):1137-44. doi: 10.1016/s0306-4522(97)00494-6.
This study examines dose-related effects on sleep produced by low-volume and low-dose carbachol microinjections in the ventral part of the cat nucleus reticularis pontis oralis. Carbachol microinjections (0.04, 0.08, 0.8 or 4 microg; volume 20 nl) in this location triggered paradoxical sleep with a very short dose-unrelated latency. The four carbachol doses effectively generated all the polygraphic and behavioral signs of paradoxical sleep when microinjected at any level within the ventral part of the nucleus reticularis pontis oralis (AP 0.5 to -3.5, L 0.5-3.5, V 3.5-5.0, on the Reinoso-Suárez atlas [Topographischer Hirnatlas der Katze (1961); Merck, Darmstadt]). The dose-related increase of total paradoxical sleep time was due to the increase in both the duration and number of paradoxical sleep episodes. This paradoxical sleep increase was associated with a dose-related decrease in the amount of time spent in both slow wave sleep and drowsiness, but not with any decrease in total wakefulness. The lengthening of the latency to slow wave sleep onset was dose related. These results show that the ventral oral pontine tegmentum is a very sensitive site for the induction and maintenance of paradoxical sleep.
本研究考察了在猫脑桥嘴侧网状核腹侧进行小容量、低剂量卡巴胆碱微量注射所产生的与剂量相关的睡眠效应。在此位置进行卡巴胆碱微量注射（0.04、0.08、0.8或4微克；体积20纳升）会引发快速眼动睡眠，且潜伏期与剂量无关，非常短暂。当在脑桥嘴侧网状核腹侧的任何水平（根据Reinoso - Suárez图谱[《猫脑图谱（1961年）》；默克公司，达姆施塔特]，前囟后0.5至 - 3.5，外侧0.5 - 3.5，腹侧3.5 - 5.0）进行微量注射时，这四种卡巴胆碱剂量均能有效产生快速眼动睡眠的所有多导睡眠图和行为体征。快速眼动睡眠总时长与剂量相关的增加是由于快速眼动睡眠发作的时长和次数均增加。这种快速眼动睡眠增加与慢波睡眠和嗜睡时长的剂量相关减少有关，但与总觉醒时间的减少无关。慢波睡眠开始潜伏期的延长与剂量相关。这些结果表明，脑桥嘴侧腹侧被盖区是诱导和维持快速眼动睡眠的一个非常敏感的部位。
