Loeb W F
Ani Lytics Inc., Gaithersburg, Maryland 20877, USA.
Toxicol Pathol. 1998 Jan-Feb;26(1):26-8. doi: 10.1177/019262339802600103.
The classical assessment of nephrotoxicity by the measurement of serum urea nitrogen and creatine is insensitive and nonspecific. Selection from a battery of clinical pathology tests allows the sensitive and specific measurement of renal dysfunction and injury. This requires the collection of urine specimens of good technical quality, with appropriate preservation. Interaction of the test article with urinary enzymes should be evaluated prior to their measurement for the assessment of tubular injury. This enables the localization and quantification of the injury within the regions of the nephron. High resolution proton nuclear magnetic resonance spectroscopy, a technique for measurement of low molecular weight metabolites, shows great promise for the evaluation of renal tubular injury in toxicologic studies.
通过测量血清尿素氮和肌酐对肾毒性进行的经典评估既不敏感也不具有特异性。从一系列临床病理检查中进行选择，可以实现对肾功能障碍和损伤的敏感且特异的测量。这需要采集技术质量良好且保存适当的尿液标本。在测量用于评估肾小管损伤的尿酶之前，应评估受试物与尿酶的相互作用。这能够在肾单位区域内对损伤进行定位和定量。高分辨率质子核磁共振波谱法是一种测量低分子量代谢物的技术，在毒理学研究中对评估肾小管损伤显示出巨大潜力。
