Bernier P J, Parent A
Laboratoire de Neurobiologie, Centre de recherche Université Laval Robert-Giffard, Beauport, Québec, Canada G1J 2G3.
J Neurosci. 1998 Apr 1;18(7):2486-97. doi: 10.1523/JNEUROSCI.18-07-02486.1998.
The distribution of neurons expressing immunoreactivity for the protein Bcl-2 was studied in the brain of squirrel monkeys (Saimiri sciureus) of various ages. Several subsets of small and intensely immunoreactive neurons displaying an immature appearance were disclosed in the amygdala and piriform cortex. The piriform cortex exhibited clusters of various forms in which Bcl-2+ neurons appeared linked to one another by their own neurites. The subventricular zone, which is known to harbor the largest population of rapidly and constitutively proliferating cells in the adult rat brain, was intensely stained, particularly at the basis of the lateral ventricle. A long and dorsoventrally oriented Bcl-2+ fiber fascicle was seen to emerge from the subventricular zone, together with numerous Bcl-2+ cells that formed a densely packed column directed at the olfactory tubercle. In adult and aged monkeys, the small and intensely labeled neurons were progressively replaced by larger and more weakly stained neurons in the amygdala and piriform cortex. In contrast, Bcl-2 immunostaining did not change with age in the subventricular zone and olfactory tubercle, the islands of Calleja of which were markedly enriched with Bcl-2. The dentate gyrus contained only a few layers of intensely labeled granule cells in juvenile monkeys, but the number of these layers increased markedly in adult and aged monkeys. These findings suggest that Bcl-2 can serve as a marker of both proliferating and differentiating neurons and indicate that such immature neurons may be much more widespread than previously thought in postnatal primate brain.
研究了不同年龄松鼠猴（松鼠猴）大脑中表达Bcl-2蛋白免疫反应性的神经元分布。在杏仁核和梨状皮质中发现了几个亚群的小而强免疫反应性神经元，呈现出不成熟的外观。梨状皮质表现出各种形式的簇，其中Bcl-2阳性神经元似乎通过它们自己的神经突相互连接。脑室下区，已知在成年大鼠脑中含有最大数量的快速和持续增殖细胞，被强烈染色，特别是在侧脑室底部。可见一条长的、背腹向的Bcl-2阳性纤维束从脑室下区出现，还有许多Bcl-2阳性细胞形成一个密集排列的柱状结构，指向嗅结节。在成年和老年猴子中，杏仁核和梨状皮质中小的、强标记的神经元逐渐被更大的、染色更弱的神经元所取代。相比之下，脑室下区和嗅结节中的Bcl-2免疫染色不随年龄变化，其中Calleja岛明显富含Bcl-2。齿状回在幼年猴子中仅含有几层强标记的颗粒细胞，但在成年和老年猴子中这些层的数量明显增加。这些发现表明，Bcl-2可以作为增殖和分化神经元的标志物，并表明这种未成熟神经元在出生后灵长类动物大脑中的分布可能比以前认为的要广泛得多。
