Johnson K A
Althouse Laboratory, Pennsylvania State University, University Park 16801, USA.
Curr Opin Biotechnol. 1998 Feb;9(1):87-9. doi: 10.1016/s0958-1669(98)80089-x.
Advances in transient-state kinetic methods of data collection by chemical-quench-flow and fluorescence stopped-flow, and in computer simulation analysis have enhanced our ability to establish reaction pathways with greater precision. These methods improve our ability to explore the functions of biologically important structures by direct measurements of single reaction steps.
化学淬灭流动法和荧光停流法在瞬态动力学数据收集方法上的进展,以及计算机模拟分析方面的进展,提高了我们更精确地建立反应途径的能力。这些方法通过直接测量单个反应步骤,增强了我们探索生物学重要结构功能的能力。