Rychlik I, Lovell M A, Barrow P A
Veterinary Research Institute, Brno, Czech Republic.
FEMS Microbiol Lett. 1998 Feb 15;159(2):255-60. doi: 10.1111/j.1574-6968.1998.tb12869.x.
A Tn3 insertion mutation was produced in the virulence plasmid of a strain of Salmonella gallinarum which conferred avirulence by parenteral and oral routes but which was also less invasive following oral inoculation. The transposon was found to have inserted near an open reading frame (ORF) with no homologies in the data banks. This ORF was adjacent to two additional ORF's with a high degree of homology of Escherichia coli genes encoding the minor structural subunits (FaeH and FaeI) of the K88 fimbria. A similar region of homology was found by DNA-DNA hybridization on the virulence plasmids of S. pullorum, S. dublin and other S. gallinarum strains but not in the plasmids of S. typhimurium, S. enteritidis or S. choleraesuis.
在鸡沙门氏菌的一个菌株的毒力质粒中产生了一个Tn3插入突变，该菌株通过肠胃外和口服途径都表现为无毒力，但口服接种后其侵袭性也较低。发现转座子插入到一个开放阅读框（ORF）附近，该开放阅读框在数据库中没有同源性。这个开放阅读框与另外两个开放阅读框相邻，它们与编码K88菌毛次要结构亚基（FaeH和FaeI）的大肠杆菌基因具有高度同源性。通过DNA-DNA杂交在鸡白痢沙门氏菌、都柏林沙门氏菌和其他鸡沙门氏菌菌株的毒力质粒上发现了类似的同源区域，但在鼠伤寒沙门氏菌、肠炎沙门氏菌或猪霍乱沙门氏菌的质粒中未发现。
