Miwa C
Gifu Prefectural Institute of Health and Environmental Science.
Kansenshogaku Zasshi. 1998 Jan;72(1):30-5. doi: 10.11150/kansenshogakuzasshi1970.72.30.
Forty four isolated strains of Coxsackievirus group A were tested for antigenic variation with antiserum against each prototype strain by neutralization test. Isolates of type 2, type 4, type 5, type 8 and type 10 of Coxsackievirus group A, were not confirmed antigenic variation by neutralization test with immune sera to the prototype virus strain. However, 57.1% of type 9 isolates of Coxsackievirus A group were confirmed as variants. In isolates of Coxsackievirus group A type 16, the isolate in 1982 was neutralized with antiserum against the prototype strain, but the isolates after 1984 were neutralized to low titer or not at all by immune sera to the prototype virus strain. Therefore, the isolated strains were tested with immune sera against the isolated strain in 1988. So that, the isolated strains in 1982 and after 1984, were neutralized with the serum against the isolate in 1988, and moreover, the neutralizing titer of prototype strain was a third of the isolate. So that, for identification of type 16 of Coxsackievirus group A, it is necessary to use the immune sera against recently isolated strains.
用针对各原型株的抗血清，通过中和试验对44株分离的A组柯萨奇病毒进行了抗原变异检测。用针对原型病毒株的免疫血清进行中和试验，未证实A组柯萨奇病毒2型、4型、5型、8型和10型的分离株存在抗原变异。然而，A组柯萨奇病毒9型分离株中有57.1%被确认为变异株。在A组16型柯萨奇病毒分离株中，1982年的分离株能被针对原型株的抗血清中和，但1984年后的分离株只能被针对原型病毒株的免疫血清低滴度中和或根本不被中和。因此，用针对1988年分离株的免疫血清对这些分离株进行了检测。结果显示，1982年及1984年后的分离株能被针对1988年分离株的血清中和，而且，原型株的中和滴度是该分离株的三分之一。所以，为鉴定A组柯萨奇病毒16型，有必要使用针对近期分离株的免疫血清。
