Karlberg A T, Skare L, Lindberg I, Nyhammar E
Department of Occupational Health, National Institute for Working Life, Solna, Sweden.
Contact Dermatitis. 1998 Jan;38(1):20-8. doi: 10.1111/j.1600-0536.1998.tb05632.x.
Reliable and uncomplicated methods for detection of free formaldehyde in products preserved with formaldehyde donors are desirable to decrease the risk of allergic contact dermatitis. The aim of this study was to develop a method that could be used in clinics and workplaces for quantification of free formaldehyde in products preserved with formaldehyde donors. The method developed is named the closed container diffusion (CCD) method. Formaldehyde in a sample is allowed to evaporate in a closed container and react with 2,4-dinitrophenylhydrazine coated on a glass fibre filter. The hydrazone formed is analyzed with HPLC. The method was tested on 3 different formaldehyde donors, imidazolidinyl urea, diazolidinyl urea and 2-bromo-2-nitropropane-1,3-diol, using 4 different cream bases. The results obtained with this method accord, with those obtained with the official method within the European Union (EU). The method is sensitive enough for analysis of patients' products and for control of labelled amounts of formaldehyde in technical products without solvent extraction. As a result of our studies, we observed a risk of exceeding the labelling limit for free formaldehyde in cosmetic products when using the highest amount of diazolidinyl urea allowed within the EU.
为降低过敏性接触性皮炎的风险，需要可靠且简便的方法来检测用甲醛供体保存的产品中的游离甲醛。本研究的目的是开发一种可用于诊所和工作场所的方法，以定量检测用甲醛供体保存的产品中的游离甲醛。所开发的方法称为密闭容器扩散（CCD）法。样品中的甲醛在密闭容器中蒸发，并与涂覆在玻璃纤维滤纸上的2,4-二硝基苯肼反应。形成的腙用高效液相色谱法分析。该方法使用4种不同的乳膏基质，对3种不同的甲醛供体（咪唑烷基脲、重氮烷基脲和2-溴-2-硝基丙烷-1,3-二醇）进行了测试。用该方法获得的结果与欧盟官方方法获得的结果一致。该方法灵敏度足以分析患者产品，并在无需溶剂萃取的情况下控制技术产品中甲醛的标注量。根据我们的研究，我们观察到，当使用欧盟允许的最大量重氮烷基脲时，化妆品中游离甲醛有超过标注限量的风险。
