Vedernikov Y P, Kravtsov G M, Postnov Y V, Saade G, Garfield R E
University of Texas Medical Branch, Department of Obstetrics and Gynecology, Galveston 77555-1062, USA.
Am J Hypertens. 1998 Jan;11(1 Pt 1):105-12. doi: 10.1016/s0895-7061(97)00362-2.
Experiments were designed to compare the contractile effect of red blood cells (RBC) on aortic rings with and without endothelium from normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats. Red blood cells of 4 week old WKY and SHR rats induced a negligible increase in tension of aortic rings, either with or without endothelium, being slightly more effective in SHR rats. However, red blood cells of 16 week old rats increased tension of WKY and SHR aortic rings, with endothelium at this age being more pronounced then red blood cells in 4 week old animals. The contractions induced by WKY and SHR red blood cells both in WKY and SHR aortic rings without endothelium at this age are significantly greater compared to the effect on aortic rings with endothelium. Red blood cell ghosts of rats of both strains increased the tension of the rings without endothelium of SHR aorta to near 50% of those induced by red blood cells, whereas they were ineffective in aortic rings without endothelium of WKY rats. Oxyhemoglobin increased the tension of 16 week SHR aortic rings both with and without endothelium, whereas the effect on the rings of WKY rats was negligible. This increase in tension was inhibited by BM 13505, nordihydroguaiaretic acid, and indomethacin in SHR rings both with and without endothelium, demonstrating an eicosanoid involvement in oxyhemoglobin-induced contractions. Hemoglobin or its metabolites may be involved in development or in maintenance of spontaneous hypertension.
实验旨在比较正常血压的Wistar-Kyoto（WKY）大鼠和自发性高血压（SHR）大鼠的红细胞（RBC）对有内皮和无内皮主动脉环的收缩作用。4周龄WKY和SHR大鼠的红细胞对有内皮或无内皮的主动脉环张力增加的影响可忽略不计，在SHR大鼠中作用稍强。然而，16周龄大鼠的红细胞可增加WKY和SHR主动脉环的张力，这个年龄段有内皮时的作用比4周龄动物的红细胞更明显。与对有内皮主动脉环的作用相比，这个年龄段WKY和SHR红细胞对无内皮的WKY和SHR主动脉环的收缩作用显著更强。两种品系大鼠的红细胞膜增加SHR主动脉无内皮环的张力至红细胞诱导张力的近50%，而对WKY大鼠无内皮主动脉环无效。氧合血红蛋白增加16周龄SHR有内皮和无内皮主动脉环的张力，而对WKY大鼠主动脉环的作用可忽略不计。在有内皮和无内皮的SHR主动脉环中，这种张力增加被BM 13505、去甲二氢愈创木酸和吲哚美辛抑制，表明类花生酸参与氧合血红蛋白诱导的收缩。血红蛋白或其代谢产物可能参与自发性高血压的发生或维持。
