Nogueira C P, Dolan R W, Gooey J, Byahatti S, Vaughan C W, Fuleihan N S, Grillone G, Baker E, Domanowski G
Department of Otolaryngology, Boston Medical Center, Massachusetts, USA.
Laryngoscope. 1998 Mar;108(3):345-50. doi: 10.1097/00005537-199803000-00007.
The authors have investigated whether genetic abnormalities in two genes, loss of heterozygosity (LOH) of p53 and amplification of the cyclin D1 gene, correlate with clinical outcome in 56 matched pairs of blood and tumor from patients with squamous cell carcinoma of the head and neck (SCCHN). Frequency of p53 LOH was 47.4%, of cyclin D1 amplification 33.9%, and of both abnormalities together 23.7%. p53 LOH was associated with T4 (P = 0.003) and stage IV (P = 0.015) tumors. Cyclin D1 amplification was associated with recurrences and/or metachronous tumors (P = 0.007). The total number of p53 and cyclin D1 abnormalities (scored as zero, one, and two) show a pattern that seems to be additive; the increase in the number of these abnormalities is associated with a proportional increase in the frequency of T4, stage IV, presence of recurrences and/or metachronous tumors, and possibly a proportional decrease in the disease-free interval in the sample. The association of the markers with recurrences and/or metachronous tumors persists if the tumor stage effect is mathematically removed. The combined analysis of the p53 and cyclin D1 abnormalities seems to be more informative than either of them individually and may have predictive value in SCCHN.
作者研究了两个基因的遗传异常，即p53杂合性缺失（LOH）和细胞周期蛋白D1基因扩增，是否与56对匹配的头颈部鳞状细胞癌（SCCHN）患者的血液和肿瘤样本的临床结局相关。p53 LOH的频率为47.4%，细胞周期蛋白D1扩增的频率为33.9%，两者同时出现异常的频率为23.7%。p53 LOH与T4期（P = 0.003）和IV期（P = 0.015）肿瘤相关。细胞周期蛋白D1扩增与复发和/或异时性肿瘤相关（P = 0.007）。p53和细胞周期蛋白D1异常的总数（分为0、1和2分）呈现出一种似乎具有累加性的模式；这些异常数量的增加与T4期频率的成比例增加、IV期、复发和/或异时性肿瘤的存在相关，并且可能与样本中无病生存期的成比例缩短相关。如果通过数学方法消除肿瘤分期的影响，这些标志物与复发和/或异时性肿瘤的关联仍然存在。p53和细胞周期蛋白D1异常的联合分析似乎比单独分析其中任何一个更具信息量，并且可能对头颈部鳞状细胞癌具有预测价值。
