Hulkkonen J, Vilpo J, Vilpo L, Hurme M
Department of Microbiology and Immunology, University of Tampere Medical School, Finland.
Br J Haematol. 1998 Mar;100(3):478-83. doi: 10.1046/j.1365-2141.1998.00595.x.
The production of the cytokines interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) in B-CLL cells from 24 patients at different stages of chronic lymphocytic B-cell leukaemia (B-CLL) was investigated in vitro. In the majority of these cases, low spontaneous IL-6 production was measured. Mitogenic stimulation with phorbol 12-myristate 13-acetate (PMA) or PMA plus interleukin-2 (IL-2) resulted in a tremendous increase in TNF-alpha and IL-6 production in cells representing early stage (Binet A) disease. In contrast, very little, if any, production took place in cells from patients with advanced stage (Binet C) B-CLL. The results from stage B patients were intermediate. The most remarkable difference was recorded in PMA-stimulated (1 ng/ml) IL-6 production. In stimulated 72 h cultures, IL-6 concentrations were 1280 +/- 1080 pg/ml for Binet A (n = 11), 757 +/- 597 pg/ml for Binet B (n = 8) and 46.0 +/- 84.0 pg/ml for Binet C (n = 5). The differences in IL-6 production between stage C v B and stage C v A were both statistically significant (P=0.025). Similar effects, but to a lesser extent, were observed in TNF-alpha production. These results suggest that the varying capacity to produce IL-6 and TNF-alpha may play a role in B-CLL progression and in clinical manifestations of the disease.
对24例处于慢性淋巴细胞B细胞白血病（B-CLL）不同阶段的患者的B-CLL细胞中细胞因子白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的产生进行了体外研究。在大多数这些病例中，检测到IL-6的自发产生量较低。用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）或PMA加白细胞介素-2（IL-2）进行有丝分裂原刺激，导致代表早期（Binet A）疾病的细胞中TNF-α和IL-6的产生大幅增加。相比之下，晚期（Binet C）B-CLL患者的细胞中几乎没有产生（如果有产生的话）。B期患者的结果处于中间水平。最显著的差异记录在PMA刺激（1 ng/ml）的IL-6产生中。在刺激72小时的培养物中，Binet A（n = 11）的IL-6浓度为1280±1080 pg/ml，Binet B（n = 8）为757±597 pg/ml，Binet C（n = 5）为46.0±84.0 pg/ml。C期与B期以及C期与A期之间IL-6产生的差异均具有统计学意义（P = 0.025）。在TNF-α产生中也观察到了类似但程度较小的影响。这些结果表明，产生IL-6和TNF-α的能力差异可能在B-CLL的进展和该疾病的临床表现中起作用。
