针对与李痘马铃薯Y病毒外壳蛋白N端区域相对应的合成多肽产生菌株特异性抗体。

Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein.

作者信息

Crescenzi A, d'Aquino L, Nuzzaci M, Ostuni A, Bavoso A, Comes S, De Stradis A, Piazzolla P

机构信息

Dipartimento di Biologia, Difesa e Biotecnologie Argo Forestali, Università degli Studi della Basilicata, Potenza, Italy.

出版信息

J Virol Methods. 1997 Dec;69(1-2):181-9. doi: 10.1016/s0166-0934(97)00158-4.

DOI:10.1016/s0166-0934(97)00158-4

PMID:9504763

Abstract

Comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (PPV-SwC) with the corresponding regions of several other PPV strains indicated that the main differences are in the N-terminal region. Polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the N-terminal region of PPV-SwC coat protein. They specifically detected PPV-SwC in different immunochemical tests.

摘要

李痘马铃薯Y病毒“甜樱桃”株系（PPV-SwC）预测的外壳蛋白氨基酸序列与其他几种PPV株系的相应区域比较表明，主要差异存在于N端区域。针对与PPV-SwC外壳蛋白N端区域1-14序列相对应的合成肽制备了多克隆抗体。在不同的免疫化学试验中，它们能特异性检测到PPV-SwC。