Kundu A K, Ohshima K, Sako N
Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Japan.
Acta Virol. 1998 Jun;42(3):167-73.
Serological differences between two zucchini yellow mosaic virus (ZYMV) isolates (ZYMV-169 and ZYMV-M) obtained from two distinct geographical locations in Japan were determined by mapping epitopes on the coat proteins (CPs) of the two isolates. A total of 45 monoclonal antibodies (MAbs) against the two isolates were produced and the epitopes on the CPs were delineated by reacting these MAbs with trypsin-treated ZYMV particles and Escherichia coli-expressed ZYMV CP fragments. Six MAbs of groups I-a and I-b, specific for ZYMV-169, recognised two epitopes in the N-terminal region at amino acids (aa) 1-28 and 6-41 of ZYMV-169 CP. Fourteen MAbs of group II, specific for ZYMV-M, recognised epitopes in the N-terminal region of ZYMV-M CP. Twenty-one MAbs of groups III-a, III-b(i), III-b(ii), and III-b(iii), reacting with both isolates, recognised four epitopes; one epitope was located in the N-terminal region at aa 6-28 and the remaining three epitopes were located in the core region at aa 42-95, 171-227 and 228-259 of ZYMV CPs.
通过绘制来自日本两个不同地理位置的两种小西葫芦黄花叶病毒（ZYMV）分离株（ZYMV - 169和ZYMV - M）外壳蛋白（CP）上的表位，确定了这两种分离株之间的血清学差异。制备了总共45种针对这两种分离株的单克隆抗体（MAb），并通过使这些MAb与经胰蛋白酶处理的ZYMV颗粒和大肠杆菌表达的ZYMV CP片段反应，描绘了CP上的表位。I - a和I - b组的6种对ZYMV - 169特异的MAb，识别ZYMV - 169 CP N端区域氨基酸（aa）1 - 28和6 - 41处的两个表位。II组的14种对ZYMV - M特异的MAb，识别ZYMV - M CP N端区域的表位。III - a、III - b（i）、III - b（ii）和III - b（iii）组的21种与两种分离株都反应的MAb，识别四个表位；一个表位位于N端区域aa 6 - 28处，其余三个表位位于ZYMV CP的核心区域aa 42 - 95、171 - 227和228 - 259处。
