Kim Y, Yeung E S
Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, 50011, USA.
Electrophoresis. 1997 Dec;18(15):2901-8. doi: 10.1002/elps.1150181530.
DNA sequencing by using pulsed-field capillary electrophoresis in a linear polymer matrix was performed in this work. The effects of waveform, frequency, modulation depth and heating were investigated. The separation performance for Sanger DNA fragments up to 1000 bp using a pulsed field was compared to isoelectric and isorheic conditions. A separation mechanism in the flexible poly(ethylene oxide) (PEO) gel was also suggested. We found that temperature change generated by either a pulsed field or ultrasound during DNA sequencing can have a deleterious effect on the separation efficiency.
本研究采用线性聚合物基质中的脉冲场毛细管电泳进行DNA测序。研究了波形、频率、调制深度和加热的影响。将使用脉冲场对长达1000 bp的桑格DNA片段的分离性能与等电和等流变条件进行了比较。还提出了柔性聚环氧乙烷（PEO）凝胶中的分离机制。我们发现，DNA测序过程中由脉冲场或超声产生的温度变化会对分离效率产生有害影响。
