Schirmacher P, Dienes H P, Moll R
Institute of Pathology, University of Mainz, Germany.
Virchows Arch. 1998 Feb;432(2):143-52. doi: 10.1007/s004280050148.
Mallory bodies (MBs) are eosinophilic cytoplasmic inclusions observed predominantly in alcoholic liver disease. Although linked to disease activity, their pathogenesis is still unclear. Since intermediate filaments (cytokeratins) are major components of MBs, their cytokeratin polypeptide composition was analysed with monospecific antibodies for cytokeratins 7, 8, 14, 18, 19, and 20 by immunohistology. MBs were identified by light microscopy and ubiquitin immunostaining. All MBs were positive for cytokeratins 8 and 18. A significant percentage of the MBs was strongly positive for cytokeratins 19 and/or 20, which are not detectable in hepatocytes of normal liver and, in the case of cytokeratin 20, in hepatocytes of diseases devoid of MBs. MBs were essentially negative for cytokeratins 7 and 14. De novo expression of cytokeratins 19 and 20 was independent of the aetiology, occurring in all MB-associated diseases analysed, and seemed to precede MB formation, since in some hepatocytes a cytoskeletal-type staining pattern for these cytokeratins was present. In hepatocellular carcinomas cytokeratins 19 and 20 were frequently detected, but their cellular distribution was less closely associated with MBs. The ectopic expression of cytokeratins 19 and 20 appears to be related to MB formation and may take part in the derangement of the intermediate filaments during MB formation.
马洛里小体（MBs）是主要在酒精性肝病中观察到的嗜酸性细胞质内含物。尽管与疾病活动有关，但其发病机制仍不清楚。由于中间丝（细胞角蛋白）是MBs的主要成分，因此通过免疫组织化学用针对细胞角蛋白7、8、14、18、19和20的单特异性抗体分析了它们的细胞角蛋白多肽组成。通过光学显微镜和泛素免疫染色鉴定MBs。所有MBs对细胞角蛋白8和18呈阳性。相当比例的MBs对细胞角蛋白19和/或20呈强阳性，在正常肝脏的肝细胞中检测不到细胞角蛋白19和/或20，就细胞角蛋白20而言，在没有MBs的疾病的肝细胞中也检测不到。MBs对细胞角蛋白7和14基本呈阴性。细胞角蛋白19和20的从头表达与病因无关，在所分析的所有与MB相关的疾病中均有发生，并且似乎先于MB形成，因为在一些肝细胞中存在这些细胞角蛋白的细胞骨架型染色模式。在肝细胞癌中经常检测到细胞角蛋白19和20，但其细胞分布与MBs的关系不太密切。细胞角蛋白19和20的异位表达似乎与MB形成有关，并且可能在MB形成过程中参与中间丝的紊乱。
