Van Eyken P, Sciot R, Desmet V J
Laboratory for Histo- and Cytochemistry, Pathology Department II, UZ St Rafaël, Catholic University of Leuven, Belgium.
Histopathology. 1988 Dec;13(6):605-17. doi: 10.1111/j.1365-2559.1988.tb02092.x.
A cytokeratin immunohistochemical study was performed on 40 liver biopsies diagnosed as alcoholic liver disease to further investigate the cytoskeletal changes occurring in this disease. On paraffin sections of 29 cases, a variable number of hepatocytes were reactive with a polyclonal antiserum that normally stains only bile ducts. Using monoclonal antibodies specific for a single cytokeratin polypeptide on cryostat sections, a variable number of hepatocytes were immunoreactive for cytokeratin no. 7 in 23 cases and also for cytokeratin no. 19 in seven cases. Both these polypeptides are restricted to bile duct cells in the normal liver. The number of hepatocytes positive for bile duct-type cytokeratins increased and their location changed with the severity of the disease. Mallory bodies were reactive with monoclonal antibodies CAM 5.2 and anti-cytokeratin no. 18 but unreactive with anti-cytokeratin no. 8. except in one case. In two cases, Mallory bodies reactive with both monoclonal antibodies anti-cytokeratin no. 7 and anti-cytokeratin no. 19 were found. These results clearly indicate that hepatocytes in alcoholic liver disease can express immunoreactivity for bile duct-type cytokeratins. Our data also demonstrate heterogeneity in the composition of Mallory bodies. Whether hepatocytes expressing bile duct-type cytokeratins are the precursors of Mallory body-containing cells is not clear at present.
对40例诊断为酒精性肝病的肝活检组织进行细胞角蛋白免疫组织化学研究，以进一步探究该疾病中发生的细胞骨架变化。在29例石蜡切片中，有数量不等的肝细胞与一种通常仅对胆管染色的多克隆抗血清发生反应。在冰冻切片上使用针对单一细胞角蛋白多肽的单克隆抗体，23例中有数量不等的肝细胞对细胞角蛋白7呈免疫反应，7例中对细胞角蛋白19也呈免疫反应。这两种多肽在正常肝脏中仅局限于胆管细胞。胆管型细胞角蛋白阳性的肝细胞数量随着疾病严重程度增加，其位置也发生改变。马洛里小体与单克隆抗体CAM 5.2和抗细胞角蛋白18发生反应，但除1例外，与抗细胞角蛋白8不发生反应。在2例中，发现马洛里小体与单克隆抗体抗细胞角蛋白7和抗细胞角蛋白19均发生反应。这些结果清楚地表明，酒精性肝病中的肝细胞可对胆管型细胞角蛋白表达免疫反应性。我们的数据还证明了马洛里小体组成的异质性。目前尚不清楚表达胆管型细胞角蛋白的肝细胞是否为含马洛里小体的细胞的前体。
