Lewis J L, Marley S B, Blackett N M, Szydlo R, Goldman J M, Gordon M Y
LRF Center for Adult Leukaemia, Royal Postgraduate Medical School, London, UK.
Cytokine. 1998 Jan;10(1):49-54. doi: 10.1006/cyto.1997.0256.
Interleukin 3 (IL-3) and stem cell factor (SCF) are both important regulators of early haemopoietic cell development. Here, we have compared their effects or the kinetics of erythroid burst formation by BFU-E in normal adult bone marrow. We grew the BFU-E in the presence of erythropoietin (Epo) alone, Epo + IL-3 or Epo + SCF and scored the numbers of subcolonies in individual bursts after 14 days. The data were plotted as the cumulative distribution of the numbers of subcolonies per erythroid burst then linearised by logarithmic transformation. Analysis of the data revealed that IL-3 increases the numbers of subcolonies in BFU-E whilst SCF increases the size of the subcolonies themselves. Experiments involving combinations of Epo + IL-3 + SCF and the delayed addition of IL-3 or SCF indicated that the actions of IL-3 and SCF are largely independent of one another. We conclude that: (1) IL-3 acts at an earlier stage of erythroid differentiation than SCF, and (2) it may be possible to classify haemopoietic growth factors according to their effects on cell kinetics in vitro.
白细胞介素3（IL-3）和干细胞因子（SCF）都是早期造血细胞发育的重要调节因子。在此，我们比较了它们对正常成人骨髓中BFU-E形成红系集落的动力学的影响。我们将BFU-E分别在单独的促红细胞生成素（Epo）、Epo + IL-3或Epo + SCF存在的条件下培养，并在14天后对单个集落中的亚集落数量进行计数。数据绘制为每个红系集落中亚集落数量的累积分布，然后通过对数转换进行线性化处理。数据分析表明，IL-3增加了BFU-E中亚集落的数量，而SCF增加了亚集落本身的大小。涉及Epo + IL-3 + SCF组合以及延迟添加IL-3或SCF的实验表明，IL-3和SCF的作用在很大程度上相互独立。我们得出以下结论：（1）IL-3在红系分化中比SCF作用于更早的阶段，（2）根据造血生长因子在体外对细胞动力学的影响对其进行分类可能是可行的。
