Zidovec S, Mazuran R
Institute of Immunology, Zagreb, Croatia.
Cytokine. 1998 Jan;10(1):70-2. doi: 10.1006/cyto.1997.0259.
The infection of human peripheral blood leukocytes (hPBL) with Sendai virus in vitro, induces the synthesis of human leukocyte interferons (IFN-alpha n3) as well as other cytokines. The authors discovered that supernatants of virus-infected hPBL also contained significant amounts of platelet-derived growth factor AB (PDGF-AB). The concentrations of immunogenic PDGF-AB in the interferon batches were determined by quantitative enzyme-linked immunosorbent assay (ELISA) before and after the inactivation of Sendai virus by acidification (pH 2) for 5 days. Immunogenic PDGF-AB molecules were detected in all interferon batches before and after acidification. The virus inactivation process caused a significant change in the content of immunogenic PDGF-AB in the interferon samples. Inactivation of the Sendai virus by acidification favoured the appearance of PDGF-AB dimers in the majority of samples.
体外将仙台病毒感染人外周血白细胞（hPBL）可诱导人白细胞干扰素（IFN-α n3）以及其他细胞因子的合成。作者发现，病毒感染的hPBL的上清液中还含有大量血小板衍生生长因子AB（PDGF-AB）。通过定量酶联免疫吸附测定（ELISA）在仙台病毒经酸化（pH 2）处理5天灭活前后，测定干扰素批次中免疫原性PDGF-AB的浓度。在酸化前后的所有干扰素批次中均检测到免疫原性PDGF-AB分子。病毒灭活过程导致干扰素样品中免疫原性PDGF-AB的含量发生显著变化。通过酸化灭活仙台病毒有利于在大多数样品中出现PDGF-AB二聚体。
