Zidovec S, Mazuran R
Institute of Immunology, Rockefellerova 2, 101 000 Zagreb, Croatia.
Cytokine. 1999 Feb;11(2):140-3. doi: 10.1006/cyto.1998.0411.
Virus infection of cell cultures induces the synthesis of various cytokines which can either inhibit or stimulate virus replication. The Sendai virus induces large quantities of biologically active interferon (IFN-alphan3) in human peripheral blood leukocytes (hPBL) in vitro, as well as many other cytokines. The supernatants of Sendai virus-infected hPBL contained biologically active IFN-alphan3, significant amounts of immunogenic IFN-gamma, monokines (IL-1alpha, IL-beta, TNF-alpha), lymphokines (IL-6, TNF-beta), growth factor (PDGF-AB) and small concentrations of IL-2 and GM-CSF. The analysis of the influence of the Sendai virus inactivation by lowering pH 2.0 on the cytokine concentrations showed that IL-1alpha, TNF-alpha, TNF-beta and IFN-gamma are susceptible to acid conditions, while IFN-alphan3, IL-1beta, IL-6 and IL-2 concentrations remained unchanged.
细胞培养物的病毒感染会诱导多种细胞因子的合成，这些细胞因子既可以抑制也可以刺激病毒复制。仙台病毒在体外可诱导人外周血白细胞（hPBL）产生大量具有生物活性的干扰素（IFN-αn3）以及许多其他细胞因子。感染仙台病毒的hPBL的上清液中含有具有生物活性的IFN-αn3、大量免疫原性IFN-γ、单核因子（IL-1α、IL-β、TNF-α）、淋巴因子（IL-6、TNF-β）、生长因子（PDGF-AB）以及低浓度的IL-2和GM-CSF。通过将pH值降至2.0来分析仙台病毒失活对细胞因子浓度的影响，结果表明IL-1α、TNF-α、TNF-β和IFN-γ对酸性条件敏感，而IFN-αn3、IL-1β、IL-6和IL-2的浓度保持不变。
