Eguchi Y, Arishima K, Morikawa Y, Hashimoto Y
J Endocrinol. 1976 Jun;69(3):427-31. doi: 10.1677/joe.0.0690427.
On day 20 of gestation, foetal rats received a subcutaneous injection of 0-01 mg oestradiol benzoate dissolved in 0-05 ml sesame oil; foetuses in other litters were given sesame oil alone. Autopsy was performed on day 22 of gestation, at delivery or at various times after birth. Gravimetric and histological observations of the adrenals from oestradiol-treated, oil-treated and intact litter-mate control foetal and neonatal rats were performed together with determination of plasma corticosterone concentrations. Activity of 3 phi-ol dehydrogenase was also examined histochemically in the adrenals from these animals. The results indicated that oestradiol benzoate when given prenatally prevented the neonatal decline of adrenal weight and adrenocortical cell size. In normal or oil-treated rats, plasma corticosterone concentration was greatly increased during delivery and 2 h after birth, declining up to 12 h after birth. Oestradiol benzoate prevented this perinatal increase and suppressed the activity of 3 phi-ol dehydrogenase in the perinatal adrenal cortices.
在妊娠第20天，给胎鼠皮下注射溶于0.05毫升芝麻油中的0.01毫克苯甲酸雌二醇；其他窝的胎鼠仅给予芝麻油。在妊娠第22天、分娩时或出生后的不同时间进行解剖。对接受苯甲酸雌二醇处理、芝麻油处理以及完整同窝对照的胎鼠和新生鼠的肾上腺进行重量测定和组织学观察，并测定血浆皮质酮浓度。还对这些动物肾上腺的3β-醇脱氢酶活性进行了组织化学检查。结果表明，产前给予苯甲酸雌二醇可防止新生鼠肾上腺重量和肾上腺皮质细胞大小下降。在正常或接受芝麻油处理的大鼠中，分娩期间和出生后2小时血浆皮质酮浓度大幅升高，至出生后12小时下降。苯甲酸雌二醇可防止围产期皮质酮浓度升高，并抑制围产期肾上腺皮质中3β-醇脱氢酶的活性。
