Shchetinina N V, Vernigora A N, Gengin M T, Firstova N V
V.G. Belinsky Penza State Pedagogical University, Pensa, Russia.
Ukr Biokhim Zh (1978). 1997 May-Jun;69(3):23-8.
Phenylmethylsulfonyl fluoride (PMSF)-inhibited carboxypeptidase (CP) is recently described exopeptidase, that cleaved arginine residues from C-terminal of enkephalin synthetic analogs. The highest enzyme activity was found in the adrenal gland and 40% lower one was revealed in the pituitary gland. A 2.5-fold lower than PMSF-inhibited CP activity in adrenal glands was present in testes. The enzyme activity in the brain regions, liver and spleen was about 2.3-fold lower than in the pituitary gland. Its activity in kidneys is insignificant. In the brain region the lowest PMSF-inhibited CP activity was observed in hippocamp and optic and auditory hills, the highest enzyme activity, in the olfactory bulbus and cerebral hemispheres. The differences between brain regions with the highest and the lowest PMSF-inhibited CP activity do not exceed 1.5 fold. The tissue and regional distribution of PMSF-inhibited CP correlated with that for CPH, enzyme of regulatory peptides processing, and it did not correlate with the distribution of lysosomal CPB. The results allow one to suggest the involvement of PMSF-inhibited CP in the processing of the precursors of biological active peptides and secretory proteins. The differences of distribution of PMSF-inhibited CP and CPH are probably connected with involvements of these enzymes in the processing of precursors of different regulatory peptides.
苯甲基磺酰氟（PMSF）抑制的羧肽酶（CP）是最近发现的一种外肽酶，它能从脑啡肽合成类似物的C末端切割精氨酸残基。在肾上腺中发现该酶活性最高，而在垂体中其活性低40%。睾丸中的PMSF抑制的CP活性比肾上腺中的低2.5倍。脑区、肝脏和脾脏中的酶活性比垂体中的低约2.3倍。其在肾脏中的活性微不足道。在脑区中，海马体以及视丘和听丘中观察到的PMSF抑制的CP活性最低，而在嗅球和大脑半球中酶活性最高。PMSF抑制的CP活性最高和最低的脑区之间的差异不超过1.5倍。PMSF抑制的CP的组织和区域分布与参与调节肽加工的酶CPH的分布相关，而与溶酶体CPB的分布无关。这些结果表明PMSF抑制的CP参与生物活性肽和分泌蛋白前体的加工。PMSF抑制的CP和CPH分布的差异可能与这些酶参与不同调节肽前体的加工有关。
