Ramírez-Zarzosa G, Gil F, Vázquez J M, Arencibia A, Latorre R, López-Albors O, Ortega A, Moreno F
Departamento de Anatomía y Embriología, Facultad de Veterinaria, Universidad de Murcia, España.
Anat Histol Embryol. 1998 Feb;27(1):21-9. doi: 10.1111/j.1439-0264.1998.tb00151.x.
Fibre-type differentiation of lateral musculature has been studied in gilthead sea bream Sparus aurata (L.) and sea bass Dicentrarchus labrax (L.) during post-larval development using ultrastructural, histochemical and morphometric techniques. The study showed three muscle layers: red, intermediate (or pink) and white. Initially, most of the red muscle showed low myosin ATPase (m-ATPase) activity fibres, whereas near the transverse septum some small high m-ATPase activity fibres appeared and later acquired a rosette aspect. Afterwards, during adult growth the red muscle showed a histochemical mosaic appearance. The pink muscle in sea bass was observed at the beginning of juvenile development by the oxidative technique (NADH-RT reaction) whereas in gilthead sea bream it was also observed at the end of larval development. The pink layer consists of high m-ATPase activity fibres. However, along the muscle development other low and moderate m-ATPase activity fibres were observed close to the red and white muscles, respectively. The white muscle of juvenile fish showed a histochemical mosaic appearance near the pink muscle. In adult specimens the mosaic white muscle spread out occupying the whole of the myotome. Morphometric analysis shows a significant increase in mean fibre diameter during post-larval development, as shown by the Student's t-test (hypertrophic growth). Skewness and kurtosis values of fibre diameters point to the generation of a new fibres from the myosatellite cells (hyperplastic growth).
利用超微结构、组织化学和形态测量技术，对金头鲷（Sparus aurata，L.）和海鲈（Dicentrarchus labrax，L.）幼体后期发育过程中侧部肌肉组织的纤维类型分化进行了研究。研究显示有三层肌肉：红色、中间（或粉色）和白色。最初，大部分红色肌肉显示出低肌球蛋白ATP酶（m-ATP酶）活性纤维，而在横隔附近出现了一些小的高m-ATP酶活性纤维，随后呈现出玫瑰花结形态。此后，在成鱼生长过程中，红色肌肉呈现出组织化学镶嵌外观。通过氧化技术（NADH-RT反应）在海鲈幼体发育初期观察到了粉色肌肉，而在金头鲷中则在幼体发育末期也观察到了粉色肌肉。粉色层由高m-ATP酶活性纤维组成。然而，在肌肉发育过程中，分别在靠近红色和白色肌肉处观察到了其他低和中等m-ATP酶活性纤维。幼鱼的白色肌肉在粉色肌肉附近呈现出组织化学镶嵌外观。在成年标本中，镶嵌状白色肌肉扩展并占据了整个肌节。形态测量分析表明，幼体后期发育过程中平均纤维直径显著增加，如学生t检验所示（肥大生长）。纤维直径的偏度和峰度值表明从肌卫星细胞产生了新的纤维（增生性生长）。
