Owen R L
University of California San Francisco, USA.
Gut. 1998 Jan;42(1):11-2. doi: 10.1136/gut.42.1.11.
The epithelium that lines the gut is impermeable to macromolecules and microorganisms, except in Peyer's patches (PP), where the lymphoid follicle-associated epithelium (FAE) contains M cells that transport antigens and microorganisms. A cultured system that reproduces the main characteristics of FAE and M cells was established by cultivation of PP lymphocytes with the differentiated human intestinal cell line Caco-2. Lymphocytes settled into the epithelial monolayer, inducing reorganization of the brush border and a temperature-dependent transport of particles and Vibrio cholerae. This model system could prove useful for intestinal physiology, vaccine research, and drug delivery studies.
除派尔集合淋巴结（PP）外，肠道内衬上皮对大分子和微生物具有不透性，在派尔集合淋巴结中，淋巴滤泡相关上皮（FAE）含有转运抗原和微生物的M细胞。通过用分化的人肠道细胞系Caco-2培养PP淋巴细胞，建立了一种再现FAE和M细胞主要特征的培养系统。淋巴细胞沉降到上皮单层中，诱导刷状缘重组以及颗粒和霍乱弧菌的温度依赖性转运。该模型系统可能对肠道生理学、疫苗研究和药物递送研究有用。
