急性胰腺炎时血清及尿液中羧肽酶B的激活肽
Activation peptide of carboxypeptidase B in serum and urine in acute pancreatitis.
作者信息
Appelros S, Thim L, Borgström A
机构信息
Department of Surgery, University Hospitals, Malmö, Sweden.
出版信息
Gut. 1998 Jan;42(1):97-102. doi: 10.1136/gut.42.1.97.
BACKGROUND
The pathophysiology of acute pancreatitis involves activation of the pancreatic proenzymes. Levels of the trypsinogen activation peptide in urine in acute pancreatitis has been shown to correlate with the severity of disease. However, this peptide is unstable in urine and, because of its low molecular mass, difficult to measure. Procarboxypeptidase B has a larger activation peptide which could be more suitable for analysis in serum and urine.
AIMS
To study the presence of the activation peptide from procarboxypeptidase B (CAPAP) in serum and urine in acute pancreatitis.
PATIENTS
Urine and serum samples were obtained within 48 hours of admittance from 40 patients with acute pancreatitis. Severity was classified retrospectively according to levels of C-reactive protein and clinical course. Thirty four patients with abdominal pain from other causes were studied as controls.
METHODS
CAPAP was purified from human pancreatic juice. Specific antibodies were obtained and a radioimmunoassay was developed.
RESULTS
Levels of CAPAP in serum and urine in acute pancreatitis correlate with the severity of the attack. CAPAP is very stable, and urine contains only CAPAP whereas, in serum, cross reacting procarboxypeptidase B is found together with CAPAP.
CONCLUSIONS
CAPAP could be a valuable tool in the diagnosis and early determination of severity in acute pancreatitis.
背景
急性胰腺炎的病理生理学涉及胰腺酶原的激活。急性胰腺炎患者尿中胰蛋白酶原激活肽的水平已被证明与疾病的严重程度相关。然而，这种肽在尿液中不稳定，并且由于其分子量低，难以测量。羧肽酶原B具有更大的激活肽，可能更适合于血清和尿液分析。
目的
研究急性胰腺炎患者血清和尿液中羧肽酶原B激活肽（CAPAP）的存在情况。
患者
40例急性胰腺炎患者在入院后48小时内采集尿液和血清样本。根据C反应蛋白水平和临床病程对严重程度进行回顾性分类。将34例因其他原因腹痛的患者作为对照进行研究。
方法
从人胰液中纯化CAPAP。获得特异性抗体并开发放射免疫测定法。
结果
急性胰腺炎患者血清和尿液中CAPAP的水平与发作的严重程度相关。CAPAP非常稳定，尿液中仅含有CAPAP，而在血清中，与CAPAP一起发现交叉反应性羧肽酶原B。
结论
CAPAP可能是急性胰腺炎诊断和早期严重程度判定的有价值工具。
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