转运体相关膜蛋白（TRAM）在新生分泌蛋白转运到内质网的过程中，调控其对细胞质溶胶的暴露。
TRAM regulates the exposure of nascent secretory proteins to the cytosol during translocation into the endoplasmic reticulum.
作者信息
Hegde R S, Voigt S, Rapoport T A, Lingappa V R
机构信息
Department of Physiology, University of California, San Francisco 94143, USA.
出版信息
Cell. 1998 Mar 6;92(5):621-31. doi: 10.1016/s0092-8674(00)81130-7.
Translocational pausing is a mechanism used by certain specialized secretory proteins whereby discrete domains of a nascent chain destined for the endoplasmic reticulum lumen are transiently exposed to the cytosol. Proteoliposomes reconstituted from total endoplasmic reticulum proteins properly assemble translocationally paused intermediates. The capacity of the translocon to correctly pause the nascent chain is dependent on a glycoprotein fraction whose active component is TRAM. In the absence of TRAM, the normally sealed ribosome-membrane junction still opens in response to a pause transfer sequence. However, nascent chain domains that are not exposed to the cytosol in the presence of TRAM are so exposed in its absence. Thus, TRAM regulates which domains of the nascent chain are visible to the cytosol during a translocational pause.
易位暂停是某些特殊分泌蛋白所采用的一种机制，通过该机制， destined for the endoplasmic reticulum lumen（此处原文似乎不完整，可能是“ destined for the endoplasmic reticulum lumen”，即“ destined for the lumen of the endoplasmic reticulum”，意为“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the 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of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the lumen of the endoplasmic reticulum”，即“ destined for the
新生链的离散结构域会短暂暴露于细胞质中。从内质网总蛋白重构的蛋白脂质体能够正确组装易位暂停中间体。转位子正确暂停新生链的能力取决于一种糖蛋白组分，其活性成分是转运体相关膜蛋白（TRAM）。在没有TRAM的情况下，正常封闭的核糖体 - 膜连接仍会响应暂停转移序列而打开。然而，在有TRAM时不暴露于细胞质的新生链结构域在没有TRAM时会暴露。因此，TRAM调节在易位暂停期间新生链的哪些结构域对细胞质可见。
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