Ratkay L G, Chowdhary R K, Iamaroon A, Richter A M, Neyndorff H C, Keystone E C, Waterfield J D, Levy J G
University of British Columbia, Vancouver, Canada.
Arthritis Rheum. 1998 Mar;41(3):525-34. doi: 10.1002/1529-0131(199803)41:3<525::AID-ART19>3.0.CO;2-I.
To study the efficacy and mechanism of local transdermal photodynamic therapy (tPDT) in rabbits with antigen-induced arthritis (AIA).
AIA in rabbits on day 14 postinduction was treated with an intravenous injection of benzoporphyrin-derivative monoacid ring A (BPD; Verteporfin) and subsequent transdermal exposure of the knee joint to light. BPD uptake and PDT-induced apoptosis of the synovium was studied applying fluorescence confocal microscopy and immunohistochemistry. The (histo)pathology of the joints was assessed at day 28.
Treatment with tPDT resulted in significant amelioration of synovial inflammation and an almost complete prevention of pannus formation and bone and cartilage destruction. BPD uptake was detectable in activated T cells and macrophages, and there was significant PDT-induced increase in the number of apoptotic cells in the synovium.
Because photodynamic therapy is both specific and noninvasive, our findings suggest that it could be used for treating arthritic joints in humans.
研究局部经皮光动力疗法（tPDT）治疗抗原诱导性关节炎（AIA）兔的疗效及机制。
诱导后第14天的AIA兔静脉注射苯卟啉衍生物单酸环A（BPD；维替泊芬），随后对膝关节进行经皮光照。应用荧光共聚焦显微镜和免疫组织化学研究BPD摄取及光动力疗法诱导的滑膜细胞凋亡。在第28天评估关节的（组织）病理学情况。
tPDT治疗导致滑膜炎症显著改善，几乎完全预防了血管翳形成以及骨和软骨破坏。在活化的T细胞和巨噬细胞中可检测到BPD摄取，且光动力疗法诱导滑膜中凋亡细胞数量显著增加。
由于光动力疗法具有特异性且无创，我们的研究结果表明其可用于治疗人类关节炎关节。
