Leistad L, Ostensen M, Faxvaag A
Rheumatology Research Laboratory, Norwegian University of Science and Technology, Faculty of Medicine, Trondheim.
Scand J Rheumatol. 1998;27(1):61-7. doi: 10.1080/030097498441191.
Cytokines are signalling glycoproteins mediating acute inflammation, chronic inflammation, and connective tissue destruction. The present study was designed to characterize the profile of cytokine message in normal human articular cartilage and from patients with rheumatoid arthritis (RA) and osteoarthritis (OA), by means of the reverse transcriptase-polymerase chain reaction (RT-PCR). Message RNA (mRNA) was extracted from fresh or frozen cartilage. The results showed expression of mRNA for IL-6, IL-6R, IL-7, IL-8, IL-10, and IL-12 (p35 and p40) exclusively in the RA cartilage. Except for mRNA for IL-8 and IL-10, no other cytokine or cytokine receptor was expressed in OA and control cartilage. mRNA for IL-1beta, IL-4, TNF-alpha, and TNFR-p75, was not detected in any cartilage sample except for one RA specimen expressing IL-1beta mRNA. However, the expression of message for pro-inflammatory cytokines was far more prominent than anti-inflammatory cytokines. This may suggest a disturbed balance of pro- and anti-inflammatory activity in RA cartilage.
细胞因子是介导急性炎症、慢性炎症和结缔组织破坏的信号糖蛋白。本研究旨在通过逆转录聚合酶链反应（RT-PCR）来表征正常人关节软骨以及类风湿性关节炎（RA）和骨关节炎（OA）患者关节软骨中细胞因子信息的特征。从新鲜或冷冻的软骨中提取信使核糖核酸（mRNA）。结果显示，IL-6、IL-6R、IL-7、IL-8、IL-10和IL-12（p35和p40）的mRNA仅在RA软骨中表达。除了IL-8和IL-10的mRNA外，OA软骨和对照软骨中未表达其他细胞因子或细胞因子受体。除了一个表达IL-1β mRNA的RA标本外，在任何软骨样本中均未检测到IL-1β、IL-4、TNF-α和TNFR-p75的mRNA。然而，促炎细胞因子信息的表达远比抗炎细胞因子突出。这可能表明RA软骨中促炎和抗炎活性的平衡受到干扰。
