Surgical Sciences and Technologies, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
Department of Pathology, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
Int J Mol Sci. 2023 Jan 20;24(3):2120. doi: 10.3390/ijms24032120.
The increase in degenerative diseases involving articular cartilage has pushed research to focus on their pathogenesis and treatment, exploiting increasingly complex techniques. Gene expression analyses from tissue are representative of the in vivo situation, but the protocols to be applied to obtain a reliable analysis are not completely cleared through customs. Thus, RNA extraction from fresh samples and specifically from musculoskeletal tissue such as cartilage is still a challenging issue. The aim of the review is to provide an overview of the techniques described in the literature for RNA extraction, highlighting limits and possibilities. The research retrieved 65 papers suitable for the purposes. The results highlighted the great difficulty in comparing the different studies, both for the sources of tissue used and for the techniques employed, as well as the details about protocols. Few papers compared different RNA extraction methods or homogenization techniques; the case study reported by authors about RNA extraction from sheep cartilage has not found an analog in the literature, confirming the existence of a relevant blank on studies about RNA extraction from cartilage tissue. However, the state of the art depicted can be used as a starting point to improve and expand studies on this topic.
退行性疾病涉及关节软骨的增加促使研究集中在其发病机制和治疗上，利用越来越复杂的技术。组织中的基因表达分析代表了体内情况，但要获得可靠分析所应用的方案尚未完全明确。因此，从新鲜样本中提取 RNA，特别是从软骨等肌肉骨骼组织中提取 RNA 仍然是一个具有挑战性的问题。本综述的目的是概述文献中描述的用于 RNA 提取的技术，突出其局限性和可能性。研究检索到 65 篇适合目的的论文。结果突出表明，由于组织来源和所用技术的不同，以及有关方案的详细信息，很难比较不同的研究。很少有论文比较不同的 RNA 提取方法或匀浆技术；作者报告的关于从绵羊软骨中提取 RNA 的案例研究在文献中没有找到类似的研究，这证实了在软骨组织的 RNA 提取研究方面存在一个相关的空白。然而，所描述的现状可以作为改进和扩展该主题研究的起点。
