Kovarik P, Hasek J, Valásek L, Ruis H
Vienna Biocenter, Institute of Biochemistry and Molecular Cell Biology of the University of Vienna, and Ludwig Boltzmann-Forschungsstelle für Biochemie, Vienna, Austria.
Curr Genet. 1998 Feb;33(2):100-9. doi: 10.1007/s002940050314.
In Saccharomyces cerevisiae cells a number of genes are required for progression through, or else to pass beyond, the G1 phase. We characterized a novel gene, RPG1, which is also involved in this phase. RPG1 is an essential gene encoding a 110-kDa evolutionarily conserved protein. Elutriated or alpha-factor-synchronized cells of the rpg1-1 temperature-sensitive mutant were arrested in the first cell cycle when shifted to a non-permissive temperature. The cells remained unbudded and neither grew nor duplicated DNA. rpg1-1 cells synchronized in S phase completed mitosis and arrested as unseparated G1 cells after a shift to a non-permissive temperature. Similarly, the asynchronous rpg1-1 cells accumulated in G1 at the non-permissive temperature, but mother and daughter cells did not separate. A bulk of Calcofluor-stained material was localized in the region adjacent to the cell septum. Our data show that Rpg1p is required for passage through the G1 phase and may be involved in growth control. Data published recently indicate that Rpg1p exhibits significant sequence similarity to a subunit of the mammalian translation initiation factor 3.
在酿酒酵母细胞中，有许多基因对于通过G1期或进入G1期之后的进程是必需的。我们鉴定了一个新基因RPG1，它也参与这一时期。RPG1是一个必需基因，编码一种110 kDa的进化保守蛋白。rpg1-1温度敏感突变体的淘洗或α因子同步化细胞在转移到非允许温度时，在第一个细胞周期中被阻滞。细胞保持无芽状态，既不生长也不复制DNA。在S期同步化的rpg1-1细胞完成有丝分裂，并在转移到非允许温度后作为未分离的G1细胞被阻滞。同样，异步的rpg1-1细胞在非允许温度下在G1期积累，但母细胞和子细胞不分离。大量经荧光增白剂染色的物质位于靠近细胞隔膜的区域。我们的数据表明，Rpg1p是通过G1期所必需的，可能参与生长调控。最近发表的数据表明，Rpg1p与哺乳动物翻译起始因子3的一个亚基具有显著的序列相似性。
