Yuh C H, Bolouri H, Davidson E H
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Science. 1998 Mar 20;279(5358):1896-902. doi: 10.1126/science.279.5358.1896.
The genomic regulatory network that controls gene expression ultimately determines form and function in each species. The operational nature of the regulatory programming specified in cis-regulatory DNA sequence was determined from a detailed functional analysis of a sea urchin control element that directs the expression of a gene in the endoderm during development. Spatial expression and repression, and the changing rate of transcription of this gene, are mediated by a complex and extended cis-regulatory system. The system may be typical of developmental cis-regulatory apparatus. All of its activities are integrated in the proximal element, which contains seven target sites for DNA binding proteins. A quantitative computational model of this regulatory element was constructed that explicitly reveals the logical interrelations hard-wired into the DNA.
控制基因表达的基因组调控网络最终决定了每个物种的形态和功能。顺式调控DNA序列中所规定的调控程序的运作本质，是通过对一个海胆控制元件进行详细的功能分析而确定的，该元件在发育过程中指导一个基因在内胚层中的表达。该基因的空间表达和抑制以及转录速率的变化，是由一个复杂且扩展的顺式调控系统介导的。该系统可能是发育性顺式调控装置的典型代表。其所有活动都整合在近端元件中，该近端元件包含七个DNA结合蛋白的靶位点。构建了这个调控元件的定量计算模型，该模型明确揭示了硬编码在DNA中的逻辑相互关系。
