Xu J, Qiu Y, DeMayo F J, Tsai S Y, Tsai M J, O'Malley B W
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Science. 1998 Mar 20;279(5358):1922-5. doi: 10.1126/science.279.5358.1922.
The in vivo biological function of a steroid receptor coactivator was assessed in mice in which the SRC-1 gene was inactivated by gene targeting. Although in both sexes the homozygous mutants were viable and fertile, target organs such as uterus, prostate, testis, and mammary gland exhibited decreased growth and development in response to steroid hormones. Expression of RNA encoding TIF2, a member of the SRC-1 family, was increased in the SRC-1 null mutant, perhaps compensating partially for the loss of SRC-1 function in target tissues. The results indicate that SRC-1 mediates steroid hormone responses in vivo and that loss of its coactivator function results in partial resistance to hormone.
通过基因靶向使SRC-1基因失活的小鼠，用于评估类固醇受体辅激活因子的体内生物学功能。虽然纯合突变体在两性中均存活且可育，但子宫、前列腺、睾丸和乳腺等靶器官对类固醇激素的反应表现出生长和发育减缓。编码SRC-1家族成员TIF2的RNA在SRC-1基因敲除突变体中表达增加，这可能部分补偿了靶组织中SRC-1功能的丧失。结果表明，SRC-1在体内介导类固醇激素反应，其辅激活因子功能的丧失导致对激素的部分抵抗。
