利用多波长动力学分析方法鉴定和表征牛肝谷氨酸脱氢酶催化的还原胺化反应中的中间复合物。
The use of multiwavelength kinetic analysis approach to identify and characterize intermediate complexes in the reductive amination reaction catalyzed by bovine liver glutamate dehydrogenase.
作者信息
Saha S K, Maniscalco S J, Fisher H F
机构信息
Laboratory of Molecular Biochemistry, Veterans Affairs Medical Center, Kansas City, MO, USA.
出版信息
Biochim Biophys Acta. 1998 Jan 15;1382(1):8-12. doi: 10.1016/s0167-4838(97)00153-2.
PMID:9507051
Abstract
A multiwavelength transient-state kinetic study of the glutamate dehydrogenase catalyzed reaction has proven that an alpha-iminoglutarate complex is an observable intermediate in the reverse direction. It also shows the existence of two enzyme-NADPH-ketoglutarate complexes, only one of which reacts with ammonia rapidly.
摘要
对谷氨酸脱氢酶催化反应进行的多波长瞬态动力学研究已证明，α-亚氨基戊二酸复合物是逆向反应中可观测到的中间体。该研究还表明存在两种酶-NADPH-酮戊二酸复合物，其中只有一种能迅速与氨发生反应。
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