Kanno M, Yagita K, Endo T, Miyata K, Araie M, Tsuru T
Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
Jpn J Ophthalmol. 1998 Jan-Feb;42(1):22-6. doi: 10.1016/s0021-5155(97)00109-3.
Although Acanthamoeba keratitis has been recognized as one of the important infectious diseases of the cornea, especially in contact lens wearers in recent years, its taxonomy has not been well established. We carried out mitochondrial DNA (mtDNA) analysis of the Acanthamoeba organisms isolated from corneal lesions in four eyes of three patients who had suffered from keratitis. The mtDNA was analyzed by restriction fragment length polymorphism (RFLP) using restriction enzymes Bg/II and EcoRI. The RFLP analyses revealed that the DNA phenotypes of the Acanthamoeba organisms were identical to those of the Ma strain in two patients and to the Castellani strain in one patient.
尽管近年来棘阿米巴角膜炎已被公认为角膜的重要传染病之一，尤其是在佩戴隐形眼镜者中，但它的分类学尚未完全确立。我们对从三名患有角膜炎的患者的四只眼中分离出的角膜病变中的棘阿米巴生物体进行了线粒体DNA（mtDNA）分析。使用限制性内切酶Bg/II和EcoRI通过限制性片段长度多态性（RFLP）对mtDNA进行分析。RFLP分析显示，两名患者的棘阿米巴生物体的DNA表型与Ma菌株相同，一名患者的与Castellani菌株相同。
