Kong Hyun-Hee, Shin Ji-Yeol, Yu Hak-Sun, Kim Jin, Hahn Tae-Won, Hahn Young-Ho, Chung Dong-Il
Department of Parasitology, Kyungpook National University School of Medicine, Taegu, Korea.
J Clin Microbiol. 2002 Apr;40(4):1199-206. doi: 10.1128/JCM.40.4.1199-1206.2002.
We applied ribosomal DNA PCR-restriction fragment length polymorphism (RFLP) and mitochondrial DNA (mtDNA) RFLP analyses to 43 Acanthamoeba environmental isolates (KA/LH1 to KA/LH43) from contact lens storage cases in southwestern Korea. These isolates were compared to American Type Culture Collection strains and clinical isolates (KA/E1 to KA/E12) from patients with keratitis. Seven riboprint patterns were seen. To identify the species of the isolates, a phylogenetic tree was constructed based on the comparison of riboprint patterns with reference strains. Four types accounted for 39 of the isolates belonging to the A. castellanii complex. The most predominant (48.8%) type was A. castellanii KA/LH2 type, which had identical riboprint and mtDNA RFLP patterns to those of A. castellanii Castellani, KA/E3 and KA/E8. The riboprint and mtDNA RFLP patterns of the KA/LH7 (20.9%) type were identical to those of A. castellanii Ma, a corneal isolate from the United States. The riboprint and mtDNA RFLP patterns of the KA/LH1 (18.6%) type were the same as those of A. lugdunensis L3a, KA/E2, and KA/E12. The prevalent pattern for each type of Acanthamoeba in southwestern Korea was very different from that from southeastern Korea and Seoul, Korea. It is noteworthy that 38 (88.4%) out of 43 isolates from contact lens storage cases of the residents in southwestern Korea revealed mtDNA RFLP and riboprint patterns identical to those found for clinical isolates in our area. This indicates that most isolates from contact lens storage cases in the surveyed area are potential keratopathogens. More attention should be paid to the disinfection of contact lens storage cases to prevent possible amoebic keratitis.
我们对来自韩国西南部隐形眼镜储存盒的43株棘阿米巴环境分离株（KA/LH1至KA/LH43）进行了核糖体DNA聚合酶链反应-限制性片段长度多态性（RFLP）分析和线粒体DNA（mtDNA）RFLP分析。将这些分离株与美国模式培养物保藏库菌株以及来自角膜炎患者的临床分离株（KA/E1至KA/E12）进行比较。观察到七种核糖体指纹图谱模式。为了鉴定分离株的种类，基于核糖体指纹图谱模式与参考菌株的比较构建了系统发育树。四种类型占属于卡氏棘阿米巴复合体的39株分离株。最主要的（48.8%）类型是卡氏棘阿米巴KA/LH2型，其核糖体指纹图谱和mtDNA RFLP模式与卡氏棘阿米巴Castellani、KA/E3和KA/E8的相同。KA/LH7（20.9%）型的核糖体指纹图谱和mtDNA RFLP模式与来自美国的角膜分离株卡氏棘阿米巴Ma的相同。KA/LH1（18.6%）型的核糖体指纹图谱和mtDNA RFLP模式与鲁氏棘阿米巴L3a、KA/E2和KA/E12的相同。韩国西南部每种棘阿米巴的流行模式与韩国东南部和首尔的非常不同。值得注意的是，来自韩国西南部居民隐形眼镜储存盒的43株分离株中有38株（88.4%）的mtDNA RFLP和核糖体指纹图谱模式与我们地区临床分离株的相同。这表明调查区域隐形眼镜储存盒中的大多数分离株是潜在的角膜病原体。应更加重视隐形眼镜储存盒的消毒，以预防可能的阿米巴性角膜炎。
