Katsaras K, Hellmann E
Zentralbl Bakteriol Orig A. 1979 Dec;245(4):512-9.
By acid hydrolysis (0.2 n HCl) it was possible to obtain extracts from nonsporulated cells of different strains of 5 species of the genus Bacillus which were composed mainly or solely of species-specific antigens. This was the result of experiments using the immunodiffusion technique after having prepared antisera from rabbits with the aid of HCl extracts. Cross-reacting antibodies were adsorbed easily. The species-specific reacting antisera revealed the existence of group- or strain-specific antigens. Therefore HCl extracts could become helpful in serotyping strains which belong to the taxa of the genus Bacillus.
通过酸水解(0.2N HCl),可以从芽孢杆菌属5个种的不同菌株的未形成芽孢的细胞中获得提取物,这些提取物主要或仅由种特异性抗原组成。这是在用HCl提取物辅助兔制备抗血清后,使用免疫扩散技术进行实验的结果。交叉反应抗体很容易被吸附。种特异性反应抗血清揭示了组特异性或菌株特异性抗原的存在。因此,HCl提取物可能有助于对芽孢杆菌属分类单元中的菌株进行血清分型。