急性肺孢子菌肺炎期间的内转录间隔区（ITSs）基因型与血液播散
ITSs genotypes and blood dissemination during acute PCP.
作者信息
Atzori C, Angeli E, Agostoni F, Mainini A, Cargnel A
机构信息
II Div. Inf. Dis., L. Sacco Hosp. Milan, Italy.
出版信息
J Eukaryot Microbiol. 1997 Nov-Dec;44(6):29S. doi: 10.1111/j.1550-7408.1997.tb05753.x.
The presence of P. carinii DNA in serum and in Peripheral Blood Mononuclear Cells (PBMC) during acute phase of PCP in AIDS patients was previously demonstrated by several authors using different specific primers. Amplification by ITSs nested PCR followed by TSO hybridization of P. carinii isolates derived from BAL and blood samples allows to compare genotypes involved in the disease and genotype-related dynamics of Pc-DNA clearance from blood during therapy. Different virulence characteristics among P. carinii genotypes could explain the various spectrum of clinical presentation (pulmonary and extrapulmonary) and susceptibility to classic antipneumocystic drugs during PCP.
此前，多位作者使用不同的特异性引物证明了艾滋病患者肺孢子菌肺炎急性期血清和外周血单个核细胞（PBMC）中存在卡氏肺孢子虫DNA。通过内转录间隔区（ITSs）巢式PCR扩增，随后对来自支气管肺泡灌洗（BAL）和血液样本的卡氏肺孢子虫分离株进行转录间隔区（TSO）杂交，能够比较参与疾病的基因型以及治疗期间血液中肺孢子虫DNA清除的基因型相关动态变化。卡氏肺孢子虫基因型之间不同的毒力特征可以解释肺孢子菌肺炎期间临床表现（肺部和肺外）的各种谱型以及对经典抗肺孢子虫药物的易感性。
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