通过内转录间隔区（ITSs）巢式聚合酶链反应（PCR）在患有卡氏肺孢子虫肺炎（PCP）的艾滋病患者的血清和外周血单核细胞（PBMC）中检测到卡氏肺孢子虫DNA。
P.carinii DNA detected by ITSs nested PCR in serum and PBMC of AIDS patients with PCP.
作者信息
Atzori C, Agostoni F, Angeli E, Orlando G, Cargnel A
机构信息
II Div. Infectious Diseases, Luigi Sacco Hospital, Milan, Italy.
出版信息
J Eukaryot Microbiol. 1996 Sep-Oct;43(5):42S. doi: 10.1111/j.1550-7408.1996.tb04978.x.
Pneumocystis carinii pneumonia (PCP) is usually diagnosed by examination of BAL, a sample often unpleasant to be collected from immunocompromised host affected by acute respiratory disease. We studied by the Internal Transcribed Spacers (ITSs) nested PCR the presence of P.carinii DNA in serum and Peripheral Blood Mononuclear Cells (PBMC) during acute episodes of PCP to test blood as a possible noninvasive diagnostic tool.
卡氏肺孢子虫肺炎（PCP）通常通过支气管肺泡灌洗（BAL）检查来诊断，对于患有急性呼吸道疾病的免疫功能低下宿主，采集该样本往往令人不适。我们采用内转录间隔区（ITSs）巢式聚合酶链反应（PCR）研究了PCP急性发作期间血清和外周血单个核细胞（PBMC）中卡氏肺孢子虫DNA的存在情况，以测试血液作为一种可能的非侵入性诊断工具。
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