Moore J, Babidge W, Millard S, Roediger W
University of Adelaide, Department of Surgery, The Queen Elizabeth Hospital, Woodville, Australia.
Dig Dis Sci. 1998 Jan;43(1):162-5. doi: 10.1023/a:1018848709769.
It has been proposed that the reduction in n-butyrate oxidation by colonic epithelial cells observed in ulcerative colitis may be related to exposure to reduced forms of sulfur derived from dissimilatory sulfate reduction by luminal microflora. This study aims to compare stool sulfide concentrations in control and colitic subjects. Control subjects had significant colorectal disease excluded by virtue of their selection. Patients with ulcerative colitis were stratified by disease extent and activity, and by salicylate drug use. Stool sulfide was measured using a direct spectrophotometric method on NaOH (free sulfide) and zinc acetate (total sulfide) stool slurries. Fifteen control and 19 colitic subjects were studied. There was no significant difference in stool sulfide between control and colitic patients (free sulfide, control = 0.52 (0.17), colitic = 0.45 (0.10), t = 0.36, P = 0.71, total sulfide, control = 1.33 (0.21), colitic = 0.96 (0.15), t = 1.44, P = 0.16). Disease extent or activity did not significantly influence stool sulfide. These results do not support a primary etiologic role for luminal sulfide in ulcerative colitis.
有人提出，在溃疡性结肠炎中观察到的结肠上皮细胞对正丁酸氧化的减少，可能与暴露于由腔内微生物异化硫酸盐还原产生的还原态硫有关。本研究旨在比较对照组和结肠炎患者粪便中硫化物的浓度。对照组因入选标准而排除了明显的结直肠疾病。溃疡性结肠炎患者按疾病范围、活动程度以及水杨酸盐药物使用情况进行分层。采用直接分光光度法对粪便NaOH悬液（游离硫化物）和乙酸锌悬液（总硫化物）中的硫化物进行测定。共研究了15名对照者和19名结肠炎患者。对照者和结肠炎患者粪便中的硫化物无显著差异（游离硫化物：对照组为0.52（0.17），结肠炎患者为0.45（0.10），t = 0.36，P = 0.71；总硫化物：对照组为1.33（0.21），结肠炎患者为0.96（0.15），t = 1.44，P = 0.16）。疾病范围或活动程度对粪便中的硫化物无显著影响。这些结果不支持腔内硫化物在溃疡性结肠炎中起主要病因作用的观点。
