Spector G J
Laryngoscope. 1976 Aug;86(8):1218-40. doi: 10.1288/00005537-197608000-00014.
The role of leucine andalanine aminopeptidases is stidued in three different biologic systems: experimental wound healing in the rat, experimental carrageenan induced intraderman granulomas in the rat, and human laryngeal carcinomas. The wound healing experiments indicate that the proliferating granulation tissue has high quantities of aminopeptidases activity which is residing primarily intracellularly in granulocytes, macrophages, mast cells fibroblasts, and new budding vessels. The quantititave levels of tissue aminopeptidases correlate positively with the degree of cellularity of the wound and fibroblastic activity. Some aminopeptidases (isoenzymes) are secreted or released by the fibroblasts in be blood serum. Starch gel electrophoretic analysis demonstrates thses with different concentrations and migration rates. Two are probably released or secreted into the serum, and th third is membranous bound in the cytoplasm (lysosomal). The carrageenan intradermal granuloma demonstrates a different inflammatory reaction which is rich in macrophages and produces a different pattern of aminopeptidases activity. Macrophages produce a high tissue level of aminopeptidase activity which is intracellular bound and not readily leached out into the serum. Gel electrophoresis studies of aminopeptidases produced by the granuloma demonstrate that the tissue bound enzyme is the main component. In addition, there appears to be a mechanism, which is not understood, for specific induction or activation of lysosomal proteolytic and carbohydrase enzymes. This mechanism is dependent on the nature of composition of the injuring agent. Laryngeal carcinomas demonstrate high tissue homogenate levels and normal serum levels of aminopeptidase activities. These enzymes are located in the tumor stroma. They are not directly related to tumor invasiveness but to the degree of stromal proliferation. The tumor stroma behaves as though it were a non-healing wound constantly secreted proteolytic enzymes (aminopeptidases). A major problem that needs to be resolved is to find the operating mechanism by which malignant cells interact with their constantly proliferating fibroblastic stroma.
大鼠的实验性伤口愈合、大鼠实验性角叉菜胶诱导的皮内肉芽肿以及人类喉癌。伤口愈合实验表明，增殖的肉芽组织具有大量的氨基肽酶活性，其主要存在于粒细胞、巨噬细胞、肥大细胞、成纤维细胞和新生血管的细胞内。组织氨基肽酶的定量水平与伤口的细胞程度和成纤维细胞活性呈正相关。一些氨基肽酶（同工酶）由成纤维细胞分泌或释放到血清中。淀粉凝胶电泳分析显示这些同工酶具有不同的浓度和迁移率。其中两种可能释放或分泌到血清中，第三种则与细胞质中的膜结合（溶酶体）。角叉菜胶皮内肉芽肿表现出不同的炎症反应，富含巨噬细胞，并产生不同的氨基肽酶活性模式。巨噬细胞产生高水平的组织氨基肽酶活性，其与细胞内结合，不易渗出到血清中。对肉芽肿产生的氨基肽酶进行凝胶电泳研究表明，组织结合酶是主要成分。此外，似乎存在一种尚不清楚的机制，用于特异性诱导或激活溶酶体蛋白水解酶和碳水化合物酶。这种机制取决于损伤剂的组成性质。喉癌显示出高组织匀浆水平的氨基肽酶活性和正常血清水平。这些酶位于肿瘤基质中。它们与肿瘤侵袭性无直接关系，而是与基质增殖程度有关。肿瘤基质的行为就好像它是一个不愈合的伤口，不断分泌蛋白水解酶（氨基肽酶）。一个需要解决的主要问题是找到恶性细胞与其不断增殖的成纤维细胞基质相互作用的运作机制。
