Spector G J
Lab Invest. 1977 Jan;36(1):1-7.
The role of leucine and alanine aminopeptidases is studied in two discrete biologic systems: experimental late stages of wound healing and carrageenan-induced intradermal granulomas in the rat. In the wound healing rats, the proliferating granulation tissue and serum have high quantities of aminopeptidase activity which correlate positively with the degree of fibroblastic activity. Starch gel electrophoresis demonstrates that in fibroblasts there are at least three groups of aminopeptidases: two isoenzymes are secreted or released into the blood serum and the third is membrane bound in the cytoplasm. The carrageenan intradermal granuloma is an inflammatory reaction rich in mononuclear phagocyte cells and macrophages. Elevated tissue levels of aminopeptidases are directly related to the degree of cellularity of the granuloma. Serum aminopeptidase levels remain normal. Starch gel electrophoretic studies of the granuloma demonstrate that the tissue-bound isoenzyme is the main component. This study indicates that fibroblasts and macrophages produce different quantities of aminopeptidase isoenzymes. In the former they are released or secreted into the serum and in the latter they are membrane bound.
大鼠伤口愈合的实验后期以及角叉菜胶诱导的皮内肉芽肿。在伤口愈合的大鼠中，增殖的肉芽组织和血清中含有大量的氨基肽酶活性，这与成纤维细胞活性程度呈正相关。淀粉凝胶电泳表明，成纤维细胞中至少有三组氨基肽酶：两种同工酶分泌或释放到血清中，第三种与细胞质中的膜结合。角叉菜胶皮内肉芽肿是一种富含单核吞噬细胞和巨噬细胞的炎症反应。氨基肽酶的组织水平升高与肉芽肿的细胞数量程度直接相关。血清氨基肽酶水平保持正常。肉芽肿的淀粉凝胶电泳研究表明，与组织结合的同工酶是主要成分。这项研究表明，成纤维细胞和巨噬细胞产生不同数量的氨基肽酶同工酶。在前者中，它们释放或分泌到血清中，而在后者中，它们与膜结合。
