Measurement of whole blood phagocyte chemiluminescence in the Wistar rat.

作者信息

Lojek A, Ciz M, Marnila P, Duskova M, Lilius E M

机构信息

Institute of Biophysics, Academy of Sciences of the Czech Republic. Brno, Czech Republic.

出版信息

J Biolumin Chemilumin. 1997 Sep-Oct;12(5):225-31. doi: 10.1002/(SICI)1099-1271(199709/10)12:5<225::AID-BIO448>3.0.CO;2-X.

The factors influencing the rat whole blood chemiluminescence (CL): concentrations of blood, luminol, zymosan or opsonized zymosan, volume of the reaction mixture, storage time of blood samples and the presence of anticoagulants were evaluated. The CL micromethod described provides a fast and sensitive tool for the determination of metabolic activity of phagocytes in the microlitre range of rat whole blood.