Miller B G, Wild J, Stone G M
Aust J Biol Sci. 1979 Dec;32(6):549-60. doi: 10.1071/bi9790549.
To examine more closely the anti-oestrogenic action of progesterone (P), its effect on various parameters in the 17 beta-oestradiol (E2)-primed uterus of the mouse, guinea pig, rabbit and ewe was studied. Changes in uterine wet weight, rate of in vitro protein synthesis, protein : DNA and RNA : DNA ratios, peroxidase activity and the level of cytosol receptors for E2 and P were measured. Considerable between-species differences in the effect of P on these parameters were observed. The anti-uterotrophic action was greater in the mouse than in the guinea pig and was not seen in the rabbit or ewe. P inhibited protein synthesis in the mouse, was without significant effect in the guinea pig and was mildly stimulatory in the rabbit and ewe. Inhibitory effects on protein : DNA and RNA : DNA ratios were substantial in the mouse, minor in the guinea pig and absent in the rabbit and ewe. Peroxidase activity was decrease in the mouse and guinea pig, essentially lacking in the rabbit and not detectable in the ewe. In all species the level of both oestrogen and progesterone cytosol receptors was decreased, although the effect on the E2 receptor was less marked in the ewe. The results indicate that in the species studied an effect of P on the replenishment of the E2 receptor is not necessarily associated with other anti-oestrogenic actions of P and argue against the concept that initial anti-oestrogenic actions of P are mediated via a specific effect on such replenishment.
为了更深入地研究孕酮（P）的抗雌激素作用，我们研究了它对经17β-雌二醇（E2）预处理的小鼠、豚鼠、兔子和母羊子宫中各种参数的影响。测量了子宫湿重、体外蛋白质合成速率、蛋白质:DNA和RNA:DNA比值、过氧化物酶活性以及E2和P的胞质溶胶受体水平的变化。观察到P对这些参数的影响在不同物种间存在显著差异。P对小鼠子宫生长的抑制作用大于豚鼠，而在兔子和母羊中未观察到这种作用。P抑制小鼠的蛋白质合成，对豚鼠无显著影响，对兔子和母羊有轻微刺激作用。对蛋白质:DNA和RNA:DNA比值的抑制作用在小鼠中显著，在豚鼠中较小，在兔子和母羊中不存在。过氧化物酶活性在小鼠和豚鼠中降低，在兔子中基本缺乏，在母羊中无法检测到。在所有物种中，雌激素和孕酮胞质溶胶受体水平均降低，尽管对E2受体的影响在母羊中不太明显。结果表明，在所研究的物种中，P对E2受体补充的影响不一定与P的其他抗雌激素作用相关，这与P的初始抗雌激素作用是通过对这种补充的特定作用介导的概念相悖。
