Sakai T, Momose-Sato Y, Sato K, Hirota A, Kamino K
Department of Physiology, Tokyo Medical and Dental University School of Medicine, Japan.
Neurosci Lett. 1998 Feb 6;242(1):1-4. doi: 10.1016/s0304-3940(98)00009-3.
Multiple-site optical recording of neural activity, using a voltage-sensitive merocyanine-rhodanine dye (NK2761) and a 12 x 12-element photodiode array, was employed to monitor the synaptic transmission in the bullfrog lumbar sympathetic ganglion. When the presynaptic nerve fibers were stimulated, the signals had two or three peaks, and their later phase was reduced in a low calcium bathing solution or in a solution containing D-tubocurarine. We conclude that the first phase of the optical signals evoked by the presynaptic stimulation corresponds to the electrical activity of the presynaptic neurons. The signals evoked by the postsynaptic stimulation showed relatively simple shape reflecting the action potential in the ganglion cells.
利用电压敏感染料（NK2761）和12×12元件光电二极管阵列对神经活动进行多部位光学记录，用于监测牛蛙腰交感神经节中的突触传递。当刺激突触前神经纤维时，信号有两个或三个峰值，并且在低钙浴液或含有筒箭毒碱的溶液中其后期阶段会减弱。我们得出结论，突触前刺激诱发的光学信号的第一阶段对应于突触前神经元的电活动。突触后刺激诱发的信号呈现出相对简单的形状，反映了神经节细胞中的动作电位。
