Sawanobori T, Hirota A, Fujii S, Kamino K
Jpn J Physiol. 1981;31(3):369-80. doi: 10.2170/jjphysiol.31.369.
The absorption signals of a merocyanine-rhodanine dye in response to action potential were measured on the bullfrog atrium. Simultaneous recording of optical signals and membrane potential showed the same time course within 15 to 30 min after exposure to Ca2+- free solution. The action spectrum of the dye exhibited a triphasic pattern with a decrease in absorption between 530 and 600 nm, an increase between 640 and 720 nm and a decrease at 750 nm. Using five photodetectors we have able to simultaneously monitor the action potential from five different regions of the preparation. The conduction velocity could be measured even in Ca2+- free solution; the velocity measured optically by the simultaneous multi-recording method was equal to that obtained with a microelectrode measurement. Thus, the optical method can validly be used to solve some problems on arrhythmia-related mechanism.
在牛蛙心房上测量了部花青罗丹宁染料对动作电位的吸收信号。光学信号和膜电位的同步记录显示，在暴露于无钙溶液后15至30分钟内，两者具有相同的时间进程。该染料的作用光谱呈现出三相模式，在530至600纳米之间吸收减少，在640至720纳米之间增加，在750纳米处减少。使用五个光电探测器，我们能够同时监测标本五个不同区域的动作电位。即使在无钙溶液中也能测量传导速度；通过同步多记录方法光学测量的速度与用微电极测量得到的速度相等。因此，光学方法可有效地用于解决一些与心律失常相关机制的问题。
